Archives
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2-Thio-dCTP for Precision DNA Assays
2026-08-21
2-Thio-dCTP enables controlled sulfur substitution in DNA for polymerase selectivity tests, site-specific DNA modification, and DNA–protein interaction studies. This practical workflow also shows how modified DNA assays can complement, but not replace, the histone-centered findings of the SCP4 chromosome-stability study.
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Tricine-SDS-PAGE Gel Preparation Kit Guide
2026-08-20
The Tricine-SDS-PAGE Gel Preparation Kit is designed to improve separation of low-molecular-weight proteins and peptides that may be poorly resolved by conventional Tris-SDS-PAGE. It is intended for controlled research workflows, including denaturing or non-denaturing electrophoresis when the sample and running conditions are configured appropriately, and is not for diagnostic or medical use.
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PTX3–TLR4/NF-κB–FGF21 Axis in Osteonecrosis
2026-08-20
The reference study identifies a PTX3–TLR4/NF-κB–FGF21 signaling axis that links impaired osteogenesis and apoptosis in glucocorticoid-induced osteonecrosis of the femoral head. Its combination of patient evidence, cellular perturbation, knockout models, pathway blockade, and downstream rescue supports PTX3 and FGF21 as mechanistically connected targets, while also defining important limits for translating the findings.
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Tioconazole Workflows for Antifungal Research
2026-08-19
Build more reproducible in vitro antifungal assays with Tioconazole by aligning solvent handling, exposure design, and ergosterol-linked readouts. This workflow also shows how the reference study’s energy-state findings can inform exploratory fungal assay stratification without overextending the evidence.
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Müller Cell PEDF Controls Retinal Neuron Survival
2026-08-19
The reference study identifies a Müller cell–mediated pathway in which opposing Ang-1 and Ang-2 signals regulate Tie-2–PI3K/Akt activity, PEDF production, and retinal neuronal survival. Its co-culture and loss-of-function design separates direct neuronal responses from glial-mediated effects, offering a useful framework for studying hypoxic retinal injury and testing pathway-selective perturbations.
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Ibrutinib, CSK Inhibition, and Atrial Fibrillation
2026-08-18
Xiao et al. identified off-target inhibition of C-terminal Src kinase (CSK), rather than Bruton tyrosine kinase inhibition itself, as the principal mechanism linking ibrutinib to atrial fibrillation. By combining mouse electrophysiology, chemoproteomics, cardiac-specific genetics, and pharmacovigilance analysis, the study provides a strong framework for distinguishing therapeutic kinase targets from cardiovascular toxicity mechanisms.
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Romidepsin (FK228) Cancer Research Workflows
2026-08-18
Romidepsin (FK228) is a selective class I HDAC probe for connecting chromatin remodeling with cell-cycle and apoptosis phenotypes. This workflow shows how to control solvent, dose, timing, target-engagement assays, and proteomic validation while using the Platycodin D study as a complementary model for mechanism-first cancer research.
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X-Gal Workflows for Cloning and Reporter Assays
2026-08-17
X-Gal converts β-galactosidase activity into a practical visual decision point for clone selection, reporter validation, and assay troubleshooting. This guide connects blue-white colony screening with activity-linked research while clearly separating established cloning uses from exploratory olfactory biology applications.
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Cytochalasin D: Actin Polymerization Inhibitor
2026-08-17
Cytochalasin D is a potent actin polymerization inhibitor used to perturb cytoskeletal dynamics, cell-cycle signaling, cancer-cell behavior, and virus-associated cellular processes. The B6645 product information reports a 25 nM IC50, while ocular nanoparticle research provides a relevant framework for testing actin dependence during cellular uptake.
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Photothermal Therapy and CD47 Blockade in OSCC
2026-08-16
A 2026 study shows that photothermal therapy (PTT) strengthens CD47 blockade in oral squamous cell carcinoma by supplying both a calreticulin-dependent “eat me” signal and improved macrophage access through extracellular matrix remodeling. The work provides a mechanistic framework for combining local thermal treatment with innate immune checkpoint inhibition in solid tumors.
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Gefitinib in Gastric Cancer Assembloid Assays
2026-08-15
Gefitinib (ZD1839) provides a mechanistically defined probe for EGFR signaling pathway inhibition in patient-derived gastric cancer assembloids. This article explains how matched tumor–stroma models can reveal response discordance, resistance biology, and assay-design requirements that conventional organoids may conceal.
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Danazol Workflows for Endocrine Research
2026-08-14
Danazol enables controlled interrogation of steroidogenesis, hypothalamic–pituitary–gonadal signaling, and androgen-responsive biology. This workflow translates a recent rat puberty model into practical cell, tissue, and translational assay decisions while emphasizing formulation, controls, and interpretation.
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SC 79: Reliable Akt Assay Design
2026-08-14
Learn how SC 79, supplied as SKU B5663, can be integrated into cell viability, proliferation, cytotoxicity, and Akt signaling experiments. This scenario-based guide covers mechanism, controls, solvent handling, metabolic-model interpretation, and practical vendor selection.
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EdU Imaging Kits (Cy3) for VSMC S-Phase Assays
2026-08-13
EdU Imaging Kits (Cy3) convert DNA replication into a bright Cy3 signal without the DNA denaturation required by many BrdU workflows. This guide shows how to apply the assay to vascular smooth muscle cell phenotypic switching, quantify S-phase entry, and troubleshoot microscopy or flow-cytometry results.
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Angiotensin I: Applied Research Workflows
2026-08-13
Turn Angiotensin I into a controllable substrate challenge for ACE activity studies, antihypertensive drug screening, and mechanistic cardiovascular research. This workflow emphasizes peptide handling, quantitative Ang II readouts, interference-aware data processing, and practical troubleshooting.